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IL-22 is a key player in the regulation of inflammation in fish and involves innate immune cells and PI3K signaling

TitleIL-22 is a key player in the regulation of inflammation in fish and involves innate immune cells and PI3K signaling
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2013
AuthorsCosta, M.M., Saraceni P.R., Forn-Cuní G., Dios S., Romero A., Figueras A., and Novoa B.
JournalDevelopmental and Comparative Immunology
Volume41
Pagination746-755
ISSN0145305X
KeywordsAdaptive Immunity, Aeromonas, Aeromonas hydrophila, Aeromonas salmonicida, animal cell, Animals, article, bone marrow cell, controlled study, developmental stage, Embryo, Fish Diseases, Fishes, Flatfishes, gene silencing, IL-22, Immunity, immunocompetent cell, immunoregulation, inflammation, Innate, innate immunity, interleukin 22, Interleukins, Janus kinase inhibitor, Mortality, nonhuman, phosphatidylinositol 3 kinase, Phosphatidylinositol 3-Kinases, PI3K, priority journal, protein expression, protein function, recombinant interleukin 22, Recombinant Proteins, signal transduction, STAT protein, turbot, unclassified drug, zebra fish, Zebrafish
Abstract

IL-22 plays a role in various disorders in mammals, including mucosal-associated infections and inflammatory diseases. No functional IL-22 studies have been conducted on non-mammals to date. In this study, recombinant IL-22 (rIL-22) from turbot was produced to investigate its effects as a bioactive molecule. The expression of several pro-inflammatory cytokines was increased after rIL-22 treatment and reduced by pre-treatment with a JAK/STAT inhibitor. The involvement of the PI3K pathway in IL-22 induction was demonstrated. rIL-22 reduced the mortality in Aeromonas salmonicida-infected turbot, while higher Aeromonas hydrophila- or LPS-induced mortality was observed when IL-22 was blocked in zebrafish embryos. IL-22 knockdown increased pro-inflammatory cytokine expression in bacteria-stimulated fish. In zebrafish, IL-22 expression was detected primarily in the myeloid innate linage. It was found during early developmental stages when the adaptive immune response is not yet functional and in rag1-/. - fish that lack an adaptive immune system. Our results clarify the conserved role of IL-22 in lower vertebrates. We suggest for the first time that IL-22 constitutes a key regulator of inflammatory homeostasis even in distant species such as teleosts, which diverged from mammals more than 350. million years ago. © 2013 Elsevier Ltd.

Notes

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84884228195&doi=10.1016%2fj.dci.2013.08.021&partnerID=40&md5=3a85377a3d0269fc6f5d9d3173f2e957
DOI10.1016/j.dci.2013.08.021
Citation KeyCosta2013746