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Bioaccumulation of antibiotics and resistance genes in lettuce following cattle manure and digestate fertilization and their effects on soil and phyllosphere microbial communities

TitleBioaccumulation of antibiotics and resistance genes in lettuce following cattle manure and digestate fertilization and their effects on soil and phyllosphere microbial communities
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2022
AuthorsCaracciolo, Anna Barra, Visca Andrea, Rauseo Jasmin, Spataro Francesca, Garbini Gian Luigi, Grenni Paola, Mariani Livia, Miritana Valentina Mazzurco, Massini Giulia, and Patrolecco Luisa
JournalEnvironmental Pollution
Keywords16S rRNA gene, Actinobacteria, animal, Animals, Anti-Bacterial Agents, antibiotic resistance, Antibiotic resistance genes, Antibiotics, antiinfective agent, article, Bacillus, Bacterial, bacterial gene, bioaccumulation, biochemistry, Biodiversity, bovine, Cattle, cattle manure, chemistry, Ciprofloxacin, Clostridia, community structure, controlled study, Degradation, Degradation kinetics, Digestate, Enrofloxacin, fertilization, fertilizer application, Fertilizers, Fluoroquinolones, Gammaproteobacteria, gene, Genes, high throughput sequencing, human, intI1 gene, Lactucum sativa, leafy vegetable, Lettuce, manure, Manures, Microbial biodiversity, Microbial community, microbiology, Microbiota, microflora, Microorganisms, mobile genetic element, nonhuman, Phyllospheres, plant growth, plant leaf, qnrS gene, quinolone derivative, Resistance genes, Rhizosphere, RNA 16S, Soil, soil amendment, soil analysis, soil fertility, Soil Microbiology, soil microflora, Soils, sul1 gene, sul2 gene, Sulfamethoxazole, Sulfur compounds, tnpA gene

The degradation and bioaccumulation of selected antibiotics such as the sulfonamide sulfamethoxazole (SMX) and the fluoroquinolones enrofloxacin (ENR) and ciprofloxacin (CIP) were investigated in soil microcosm experiments where Lactuca sativa was grown with manure or digestate (1%) and spiked with a mixture of the three antibiotics (7.5 mg/kg each). The soil, rhizosphere and leaf phyllosphere were sampled (at 0 and 46 days) from each microcosm to analyze the antibiotic concentrations, main resistance genes (sul1, sul2, qnrS, aac-(6’)-Ib-crand qepA), the intI1and tnpA mobile genetic elements and the microbial community structure.Overall results showed that SMX and CIP decreased (70-85% and 55-79%, respectively), and ENR was quite persistent during the 46-day experiment. In plant presence, CIP and ENR were partially up-taken from soil to plant. In fact the bioaccumulation factors were > 1, with higher values in manure than digestate amended soils. The most abundant gene in soil was sul2 in digestate- and aac-(6’)-Ib-cr in the manure-amended microcosms. In soil, neither sulfamethoxazole-resistance (sul1 and sul2), nor fluoroquinolone-resistance (aac-(6’)-Ib-cr, qepA and qnrS) gene abundances were correlated with any antibiotic concentration. On the contrary, in lettuce leaves, the aac-(6’)-Ib-cr gene was the most abundant, in accordance with the fluoroquinolone bioaccumulation. Finally, digestate stimulated a higher soil microbial biodiversity, introducing and promoting more bacterial genera associated with antibiotic degradation and involved in soil fertility and decreased fluoroquinolone bioaccumulation. © 2022 Elsevier Ltd


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Citation Keycaracciolo2022bioaccumulation