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An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene.

TitleAn evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene.
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication1988
AuthorsGiuliano, Giovanni, Pichersky E., Malik V.S., Timko M.P., Scolnik P.A., and Cashmore A.R.
JournalProceedings of the National Academy of Sciences of the United States of America
Keywordsarticle, Base Sequence, DNA binding protein, DNA-Binding Proteins, Genes, genetics, homeobox, molecular genetics, Molecular Sequence Data, nucleotide sequence, Plant, Plants, protein binding, Ribulose-Bisphosphate Carboxylase, ribulosebisphosphate carboxylase, vegetable, Vegetables

A protein factor, identified in nuclear extracts obtained from tomato (Lycopersicon esculentum, Solanaceae) and Arabidopsis thaliana (Brassicaceae) seedlings, specifically binds upstream sequences from the plant light-regulated gene family encoding the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (RBCS). RBCS upstream sequences from tomato, pea (Pisum sativum, Leguminosae), and Arabidopsis are recognized by the factor. The factor recognition occurs via a short conserved sequence (G box) whose consensus sequence is 5'-TCTTACACGTGGCAYY-3' (where Y is pyrimidine). This sequence is distinct from the GT motif described previously in RBCS promoters. Two other conserved sequences, showing a lesser degree of evolutionary conservation, are found upstream of the G box but do not bind to the G box binding factor (GBF). Twelve nucleotides within the G box are sufficient for the formation of a stable DNA-GBF complex. GBF is found in both light-grown and dark-adapted tomato leaf extracts, but it is present in greatly reduced amounts in root extracts.


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Citation KeyGiuliano19887089