An EST database from saffron stigmas

TitleAn EST database from saffron stigmas
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2007
AuthorsD'Agostino, N., Pizzichini D., Chiusano M.L., and Giuliano Giovanni
JournalBMC Plant Biology
Volume7
ISSN14712229
Keywordsarticle, Complementary, complementary DNA, computer interface, Crocus, Crocus sativus, Databases, DNA, expressed sequence tag, Expressed Sequence Tags, gene, Genes, Genetic, genetic database, genetics, Internet, Iridaceae, Plant, User-Computer Interface
Abstract

Background. Saffron (Crocus sativus L., Iridaceae) flowers have been used as a spice and medicinal plant ever since the Greek-Minoan civilization. The edible part - the stigmas - are commonly considered the most expensive spice in the world and are the site of a peculiar secondary metabolism, responsible for the characteristic color and flavor of saffron. Results. We produced 6,603 high quality Expressed Sequence Tags (ESTs) from a saffron stigma cDNA library. This collection is accessible and searchable through the Saffron Genes database http://www.saffrongenes.org. The ESTs have been grouped into 1,893 Clusters, each corresponding to a different expressed gene, and annotated. The complete set of raw EST sequences, as well as of their electopherograms, are maintained in the database, allowing users to investigate sequence qualities and EST structural features (vector contamination, repeat regions). The saffron stigma transcriptome contains a series of interesting sequences (putative sex determination genes, lipid and carotenoid metabolism enzymes, transcription factors). Conclusion. The Saffron Genes database represents the first reference collection for the genomics of Iridaceae, for the molecular biology of stigma biogenesis, as well as for the metabolic pathways underlying saffron secondary metabolism.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-38849203752&doi=10.1186%2f1471-2229-7-53&partnerID=40&md5=977e18d14b67eb224fe8a377bc7f1c6d
DOI10.1186/1471-2229-7-53