| Title | Virus-induced gene silencing as a tool to study Tomato Fruit Biochemistry |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2016 |
| Authors | Fantini, E., and Giuliano Giovanni |
| Journal | Methods in Molecular Biology |
| Volume | 1363 |
| Pagination | 65-78 |
| ISSN | 10643745 |
| Keywords | Agrobacterium tumefaciens, bacterial growth, bioinformatics, gene expression regulation, gene silencing, gene transfer, Gene Transfer Techniques, gene vector, genetic procedures, Genetic Vectors, genetics, Lycopersicon esculentum, metabolism, molecular cloning, nonhuman, Plant, plant gene, plant virus, Plant Viruses, RNA Interference, Tobacco rattle virus, Tomato, virus induced gene silencing |
| Abstract | Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens © Springer Science+Business Media New York 2016. |
| Notes | cited By 10 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84947746441&doi=10.1007%2f978-1-4939-3115-6_7&partnerID=40&md5=db579b27aaaf4a74a305cbd583351f43 |
| DOI | 10.1007/978-1-4939-3115-6_7 |
| Citation Key | Fantini201665 |
